Comparison of the effectiveness of 3 methods of detecting Blastocystis hominis

作者：LU Zuo-chao · 发表于：Journal of Pathogen Biology · 年份：2013 · 被引用次数：1 · 研究领域：Parasitic Infections and Diagnostics

Objective To compare the effectiveness of 3 methods of detecting Blastocystis hominis.Methods Four hundred fecal specimens were collected from patients with diarrhea and 394 specimens were collected from healthy controls.Saline smears,iodine staining,and a modified acid-ether sedimentation technique were used to detect B.hominis and its morphology was observed.Results The 3 methods detected B.hominis in patients with diarrhea at rates of 11.5%,12.75%,and 20.75%.The modified acid-ether sedimentation technique had a higher rate of detecting B.hominis than did the other two methods(χ2=15.804,P0.05).The technique also had a significantly higher detection threshold(parasite density) than the other two methods(χ2=144.715,P0.05).In the healthy controls,the modified acid-ether sedimentation technique had the highest rate of detecting B.hominis(1.52%) and the highest detection threshold(parasite density).The most common morphology was the vacuolar form,followed by the granular form.Conclusion An appropriate method should be chosen based on practical needs and detection conditions.