Randomly amplified polymorphic DNA study of Candida albicans.

作者：Hongxu Xu, Jianyu Huang, Yingpeng Cui, Cong Xu, Xiaohe Zheng · 发表于：Zhongguo yiyuan ganranxue zazhi · 年份：2010 · 研究领域：Antifungal resistance and susceptibility、Fungal Infections and Studies、Probiotics and Fermented Foods

OBJECTIVE To genotype the clinical Candida albicansisolates by randomly amplified polymorphic DNA(RAPD) method,study molecular epidemiology of C.albicans infection and discuss possible relationship between RAPD patterns and antifungal test results.METHODS Thirty C.albicansisolates with corresponding patients information and antifungal drug susceptibility test results were collected.All the isolates were genotyped by RAPD fingerprinting by employing primer OPE-03.Cluster analysis was performed by Neighbor Joining and unweighted pair-group method using anarithmetic average methods.RESULTS Primer OPE-03 was suitable for fingerprinting analysis,yielding well-resolved banding patterns.Generally,the isolates could be divided into three clusters.CONCLUSIONS RAPD fingerprinting can be used to genotype C.albicans.The RAPD patterns of C.albicansmay be related to the origins of fungi,which also related to drug resistance.