The Choices and Applications of Fluorescent Probes in the Laser Scanning Confocal Microscopy

作者：Huo Xia, Xijin Xu, Yaowen Chen · 年份：1999 · 研究领域：Photoacoustic and Ultrasonic Imaging、Optical Imaging and Spectroscopy Techniques、Advanced Fluorescence Microscopy Techniques

The laser scanning confocal microscopy is a newest biological analysis instrument to detect fluorescent messages. It is not only widely applied in fluorescent qualitative and quantitative measurement,but also applied in kinetic signal monitoring of living cells, optical sectioning of cell and tissue, 3D reconstruction, confocal image analysis, fluorescence recovery after photobleading (FRAP), laser microdissetion, etc. In present paper, the common examining items of the laser scanning confocal microscopy as well as the choices and applications of fluorescent probes will be discussed.