Diagnostic value of IL-6,IL-8,CD64 and CD11b detection in neonatal infectious diseases

作者：Zheng Jin · 发表于：Laboratory Medicine and Clinic · 年份：2015 · 研究领域：Neonatal and Maternal Infections、Neonatal and fetal brain pathology、Immune Response and Inflammation

Objective To explore the value of the interleukin-6(IL-6),IL-8,CD64 and CD11bdetection in early diagnosis of neonatal infection.Methods The neonates in the pediatric and obstetric departments of our hospital from Mar.2013 to Mar.2014 were selected and divided into the infection group(n=50),non-infection control group(n=30)and healthy control group(n=50).The IL-6and IL-8levels were detected by the enzyme-linked immunosorbent assay(ELISA),the expression of peripheral blood neutrophil CD64 and CD11bwas detected by the whole blood cytometry trichromatic fluorescent marker method.The levels and positive rate of IL-6,IL-8,CD64 and CD11b were compared among the three groups.Results The serum levels of IL-6and IL-8were(187.3±25.1)ng/L and(1.05±0.32)pg/mL in the infection group,(60.9±5.2)ng/L and(0.35±0.12)pg/mL in the non-infected control group and(53.2±9.3)ng/L and(0.30±0.05)pg/mL in the healthy control group,the infection group was higher than the non-infection group and the control group respectively,the differences were statistically significant(P0.05).The expression levels of peripheral blood neutrophil CD64 and CD11bin the infection group were higher than those in the non-infected group and the healthy control group with statistically significant differences(P0.05).Conclusion Determining the serum IL-6,IL-8levels and the expression levels of peripheral blood neutrophil CD64 and CD11bin neonates might diagnose the early infectious disease more timely,accurately and sensitively.