Regulative effects of Liuwei Dihuang decoction and its decomposed recipes on the immune function of senescence-accelerated mice.

作者：Sidi Li, Ning Jiang, Xiaorui Zhang, Zhou Wenxia, Yongxiang Zhang · 发表于：Guoji yaoxue yanjiu zazhi · 年份：2010 · 研究领域：Immune Response and Inflammation、Immune cells in cancer

Objective To investigate the effects of Liuwei Dihuang decoction (LW),its San-bu (three tonics) and San-xie (three eliminators) ingredients on regulating the immune function of senescence-accelerated mice(SAM). Methods SAM-prone(SAMP)8 mice were orally administrated with LW (10 g/kg),San-bu (6.4 g/kg) and San-xie (3.6 g/kg) respectively,once per day for 60 days,while SAM-resistant(SAMR)1 mice administrated with distilled water as control group. 3H-TdR incorporation was applied for detecting splenocyte proliferation. Flow cytometry(FCM)was used to observe the percentage of CD3+,CD4+,CD8+ and CD19+ lymphocytes in the spleens. Results Compared with SAMR1 group,the splenocyte proliferation induced by ConA an LPS,percentages of CD3+,CD4+,CD8+ and CD19+ lymphocytes and ratios of CD3+/CD19+ and CD4+/CD8+ in spleens decreased,while percentage of CD19+ lymphocytes increased in SAMP8 group. After administration of LW,San-bu and San-xie,the above indexes were improved to various degrees. LW showed better effects on improving LPS-induced splenocyte proliferation,percentages of CD3+,CD19+ and CD4+ lymphocytes,and the ratios of CD3+/CD19+ and CD4+/CD8+ than San-bu and San-xie. San-bu showed better effect on improving ConA-induced splenocyte proliferation than LW and San-xie,and better effect on improving the percentages of CD3+,CD19+ and CD4+ lymphocytes in the spleens and the ratios of CD3+/CD19+ than San-xie,while San-xie had better effect on regulating CD4+/CD8+ ratio than San-bu. Concl...