[Determination of aflatoxins in peanut by high performance liquid chromatography using immunoaffinity column clean-up and on-line electrochemical derivatization].

作者：P Zhang, Y B Zhang, Wen-Song Zhao, Ying-Huan Li · 发表于：PubMed · 年份：2000 · 被引用次数：2 · 研究领域：Analytical Chemistry and Chromatography、Biopolymer Synthesis and Applications

A method for the determination of aflatoxin B1, B2, G1 and G2 in peanut by high performance liquid chromatography using immunoaffinity column clean-up and on-line electrochemical derivatization was developed. Sample was extracted with 80% methanol and the extract was passed through the IAC. Aflatoxins were retained by the monoclonal in the IAC and eluted with actonitrile. AFT B1 and G1 were derivatized with on-line electrochemical equipment Kobra Cell. The four toxins can be separated in 13 minutes and the detection limit was 0.1 microgram/kg for each toxin. The method has been applied to the determination of peanut sample. The relative standard deviation was 9.2%-15% and the average recovery of AFT spiked at 0.5-9.0 micrograms/kg levels was in the range of 74.8%-97.3%.