Molecular characterization of microbial populations in anaerobic PCP-degrading bioreactor

作者：Quan Tang · 发表于：Acta Scientiae Circumstantiae · 年份：2005 · 被引用次数：4 · 研究领域：Microbial Community Ecology and Physiology、Anaerobic Digestion and Biogas Production、Microbial bioremediation and biosurfactants

The dynamics of spatial and temporal distribution of microbial populations during granulation of anaerobic sludge in PCP(pentachlorophenol) degrading bioreactor was investigated by PCR-DGGE. The anaerobic granular sludge was obtained in the expanded granular sludge bed (EGSB) reactor increased PCP loading. Changes of the eubacterial and archaeal community structure were observed during the sludge granulation process. On the basis of Dice Coefficient (C_s), similarity analysis of the DGGE band patterns indicated that the composition of microbial community at different layers of the reactor exhibited a moderate shift during the stable phase, C_s between the top and bottom layers were 71.4～77.6 for the eubacteria and 70.4～76.7 for the archaea. Whereas the significant spatial variations of the microbial populations in the reactor occurred in the case of PCP shocking\|loadings, and C_s between the top and the bottom layer was sharply decreased to 43.3 and 35.2 for eubacterial and archaeal communities respectively. Sequence analysis of successfully retrieved DGGE bands showed that the presence of an uncultured-able PCP-dechlorinating eubacteria. For the archaea domain, the results indicated the predominance of methanogens closely related to Methanosaeta concilii,Methanobacterium sp.,Methanobrevibacter sp. and Methanothrix soehngenii.