A Reversed-phase High Performance Liquid Chromatography(RP-HPLC) Procedure for Determination of Four Main Components in Boea hygrometrica(Bunge) R. Brown

作者：Zhiyi Feng · 发表于：Zhōnghuá yàoxué zázhì · 年份：2011 · 被引用次数：2 · 研究领域：Natural product bioactivities and synthesis、Phytochemistry and Biological Activities、Biological Activity of Diterpenoids and Biflavonoids

OBJECTIVE To develop a reversed-phase high-performance liquid chromatography(RP-HPLC) procedure for simultaneous determination of one phenylethanoid glycosides(PGs) and three flavonoid C-glycosides(FC-GsⅠ,FC-GsⅡ and FC-GsⅢ) in Boea hygrometrica(Bunge) R.Brown.METHODS The detection wavelength and chromatographic separation condition were optimized.ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm) was used.The 4 main components were separated with acetonitrile: tetrahydrofuran(10∶1)-0.02% phosphate in 45 min with good resolution(Rs1.5).The components were detected at 332 nm.RESULTS All correlation coefficients were greater than 0.999 0,and the linear ranges were 0.4-3.2 μg(PGs),0.32-2.56 μg(FC-GsⅠ),0.4-3.2 μg(FC-GsⅡ) and 0.46-3.52μg(FC-GsⅢ),respectively.The average recoveries were greater than 98.9%,and the relative standard deviations(RSD) were less than 2.08%(n=6).CONCLUSION The method is rapid,convenient and accurate,which can be used as the quality control method for Boea hygrometrica(Bunge) R.Brown.