Preparation of PH-Sensitive Procationic Liposomes and Tumor Distribution Study in Mice

作者：Peipei Rong, Song Jinchun · 发表于：Zhōnghuá yàoxué zázhì · 年份：2012 · 研究领域：Nanoparticle-Based Drug Delivery、RNA Interference and Gene Delivery、Nanopore and Nanochannel Transport Studies

OBJECTIVE The pH-sensitive procationic liposomes were prepared,with hydroxycamptothecin(HCPT) as model drug.METHODS The cationic liposomes were prepared by film dispersion method.The encapsulation efficiency was determined after removing unentrapped HCPT by Sephadex-G50 chromatographic column.Carboxymethyl chitosan was used to coat the cationic liposomes,then the liposomes were extruded through high pressure homogenizer.Zetasizer was used to determine the zeta potential,average size of the HCPT liposomes.pH sensitivity analysis was made through experiments of red blood cell hemolysis and tumor distribution is analysised.RESULTS The size of liposomes was around 96 nm and drug encapsulation efficiency was 78.5%.The membrane fusion of red blood cells with procationic liposomes was significantly different between pH 6.5 and pH 7.4 and the in vivo experiments in tumor-bearing mice suggested that they had a high tumor distribution.CONCLUSION The pH-sensitive procationic nanoliposomes were easy to prepare and were expected to be a promising anti-tumor drug delivery system.