[The effect of hypoxia preconditioning no binding activity of HIF-1 on the HRE with EPO in the hippocampus of mice].

作者：Guo Shao, Weihua Zhou, Cui-Ying Gao, Ran Zhang, Guowei Lu · 发表于：PubMed · 年份：2007 · 被引用次数：1 · 研究领域：Cancer, Hypoxia, and Metabolism、Erythropoietin and Anemia Treatment、Plant-based Medicinal Research

AIM: To observe change of binding activity of HIF-1 with erythropoietin (EPO) hypoxia response element (HRE) in the hippocampus of mice preconditioned to hypoxia and explore relationship between the changes and the preconditioning. METHODS: The hippocampus was removed from mice exposed to hypoxia for 0 run (control group), 1 run (H1 group) and 4 runs(H4 group). Electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP)and real time PCR were used to detect the change of activity of HIF-1 on HRE of EPO. RESULTS: Both in vitro and in vivo binding tests showed that the HIF-1 DNA-binding activities were increased in group H1 and markedly increased in group H4. CONCLUSION: The increase of HIF-1 and HRE of EPO binding activities is thought be involved in hypoxic preconditioning.