Concentratin change of chemotherapeutic agents in plasma and tissue after intraarterial and intravenous injection Gemcitabine and Cisplatin

作者：Cao Ju · 发表于：China Medical Herald · 年份：2014 · 研究领域：Cancer Treatment and Pharmacology、Cancer, Hypoxia, and Metabolism、Angiogenesis and VEGF in Cancer

Objective To study the drug concentration change of chemotherapeutic agents in plasma and tissue after arterial and intravenous injection. Methods Gemcitabine and Cisplatin were injected into the 40 mature nude rats with xenografted tumor. The rats were divided into vein injection group and artery injection group randomly. The rats were given Gemcitabine and Cisplatin respectively. Blood samples were collected at 5, 10, 20, 40, 80, 120, 360, 720 min after injection and the tumor tissue specimens were collected at 10, 40, 120, 720 min after injection. Gemcitabine concentration in plasma and tumor tissues were determined by high performance liquid chromatography(HPLC) method,while Cisplatin was determined by inductively coupled plasma-mass spectrometry(ICP-MS)method. The data were analyzed by the pharmacokinetic program. Results Regular concentration change of the three drugs in plasma and tissues were observed after the intravenous and arterial injection, which met the two-compartment model. The pharmacokinetic parameters of the two drugs after intravenous and arterial injection were different. The peak concentration in plasma of artery injection group [Gemcitabine:(20.84 ±10.11) μg/m L; Cisplatin:(15.13 ±7.12) μg/m L]were lower than those of the vein injection group [(Gemcitabine:(28.96 ±7.02) μg/m L; Cisplatin:(21.64±9.72) μg/m L],the peak concentration in tissues of the arterial injections group [Gemcitabine:(20.18±9.43) μg/m L; Cisplatin:(6.98±0.31) μg/m L] were higher tha...