Effects of arsenic trioxide on QT interval and L-type calcium channel protein mRNA expression

作者：Rong Huo · 发表于：Harbin Yike Daxue xuebao · 年份：2007 · 被引用次数：1 · 研究领域：Retinoids in leukemia and cellular processes

Objective To observe the effect of arsenic trioxide(As2O3) on QT interval of guinea pig electrocardiogram and investigate the effect of As2O3 on the L-type calcium channel mRNA expression in the cardiomyocytes of guinea pig.Methods The guinea pigs were divided into 4 groups randomly:control group,high dose group,middle dose group and low dose group.The experiment guinea pigs were given 1.6mg/kg(high dose group),0.8mg/kg(middle dose group) and 0.4mg/kg(low dose group) of As2O3,respectively.The electrocardiograms of those guinea pigs were measured and the corrected QT intervals(QTc) were cacaulated and compered.Total RNAs were extracted from the cardiomyocytes of the guinea pigs.RT-PCR was used to study the effects of As2O3 on L-type calcium channel mRNA expression in cardiomyocytes of guinea pigs.Results As2O3 at concentrations of 0.8mg/kg and 1.6mg/kg prolonged the QTc from(324±20) ms to(368±29) ms(P0.01),and(388±31) ms(P0.01),respectively.The RT-PCR studies showed that 1.6mg/kg of As2O3 enhanced the L-type calcium channel mRNA expression(compared with control groups,P0.01).Conclusion As2O3 could prolong the QT interval of guinea pig cardiomyocyte.The possible mechanism was associated with the upregulation of the L-type calcium channel mRNA expression due to the exposure to As2O3.