Detection of bovine viral diarrhea virus as contaminant in classical swine fever virus live vaccine with RT-PCR

作者：Qingchun Shen · 发表于：Zhongguo shouyi zazhi · 年份：2010 · 被引用次数：7 · 研究领域：Animal Disease Management and Epidemiology、Animal Virus Infections Studies、Viral gastroenteritis research and epidemiology

In this study,RT-PCR was established for the detection of bovine viral diarrhea virus as contaminant in classical swine fever virus live vaccine.Out of 23 samples detected with the established RT-PCR,5 were contaminated with BVDV.The sequences were analyzed by DNAStar and compared with the reference strains.The homology of the target BVDV gene sequence was 83.2%～83.5% with BVDV Oregon C24V(AF091605) and 86.4%～86.7% with NADL(M31182) respectively.