Tumor-Targeted BikDD Gene Therapy Inhibits Lymphoma Cells Growth

作者：Cao Ju · 年份：2015 · 研究领域：Virus-based gene therapy research、CAR-T cell therapy research、CRISPR and Genetic Engineering

Objective To construct a Lymphoma specific targeted gene therapy vector S-VISA-BikDD,composed of mutant Bik(BikDD),the survivin promoter,and the VP16-GAL4-WPRE integrated systemic amplifier system(VISA),to investigate its role of growth inhibition in Lymphoma cell lines.Methods S-VISA-BikDD was constructed by the way of gene recombination technology.The lymphoma cell lines Raji,Ramos and U937 were transfected with S-VISA-BikDD.Western blot was used to assess the different expression of Bik in lymphoma cell 1 ines before and after the transfection was conducted.The flow cytometry was carried out to detect pro-apoptotic effects and the MTT assay was carried out to detect growth inhibition in lymphoma cell lines.Results S-VISABikDD was proved to be constructed correctly by PCR and enzyme digestion.The expression level of BikDD in lymphoma cells was enhanced after the transfection was performed.It enhanced cell apoptosis and inhibited cell proliferation in lymphoma cells.Conclusion S-VISA-BikDD is a potent Lymphoma Gene Therapy vector,which provides a new method and target for further study of lymphoma gene therapy.