Determination of related substances in furazolidone by gradient elution HPLC with double UV wavelengths

作者：XU Yi-quan · 年份：2011 · 研究领域：Analytical Chemistry and Chromatography、Analytical Methods in Pharmaceuticals

Objective:To establish a method for determination of 5-nitro-2-furaldehyde diacetate and other related substances in furazolidone.Methods:An Alltima C18 column(150 mm×4.6 mm,5 μm) was adopted.The mobile phase was water-acetonitrile with gradient elution[0 min(90% water)→10 min(50% water)→20 min(95% water)→30 min(95% water)→35 min(90% water)];5-nitro-2-furaldehyde diacetate and other related substances were determined with double UV wavelengths,which were 303 nm for 5-nitro-2-furaldehyde diacetate and 210 nm for other related substances.Results:The resolution between furazolidone peak and other peaks was good.The linear range of 5-nitro-2-furaldehyde diacetate was 0.2516-3.744 μg·mL-1 and the average recovery(n=9) was 100.7%;The detection limits of furazolidone and 5-nitro-2-furaldehyde diacetate were 1.53 ng and 0.20 ng,respectively.The test solution was stable within 8 h.Conclusion:The method is specific,convenient,accurate,sensitive,and can be used for the quality control of furazolidone.