Research on the protective mechanism of DL-3-n-butylphthalide on rat bone marrow stem cells under oxidative stress injury induced by hydrogen peroxide

作者：Ding Xin-sheng · 发表于：Journal of Apoplexy and Nervous Diseases · 年份：2011 · 被引用次数：1 · 研究领域：Neurological Disease Mechanisms and Treatments、Traditional Chinese Medicine Analysis、Advanced Computing and Algorithms

Objective To explore the possible protective mechanism of DL-3-n-butylphthalide(NBP) on rat bone marrow stem cells(rBMSCs) under oxidative stress induced by hydrogen peroxide(H2O2).Methods The oxidative stress of rBMSCs were induced by H2O2.The experiment was carried out on six groups:control group(without the application of H2O2 or NBP),model group(cells were administrated by H2O2 without NBP pretreatment),NBP pretreated groups(cells were pretreated with different doses of NBP as 0.1μmol,1μmol,10μmol and 100μmol for 24h,then administrated with 600 μmol H2O2 for 4h).MTT analysis was used to detect the cell viability of all the groups.The rate of cell apoptosis was observed by flow cytometry analysis.The expression level of protein Caspase-3 was detected by Western blot.Results Compared with model group,MTT and cytometrical analysis showed that pretreatment with NBP could protect rBMSCs from oxidative stress dose-dependently.Up-regulation of protein Caspase-3 induced by H2O2 was significantly reduced by NBP pretreatment.Conclusion NBP has protective effects on rBMSCs challenged by H2O2.The protective mechanism may be related with the antiapoptotic effects provided by NBP.