Specific quantification of mouse and human keratin proteins by radioimmunoassay

作者：Stuart H. Yuspa, M C Poirier, J R Harness, D R Olsom, Peter M. Steinert · 发表于：Biochemical Journal · 年份：1980 · DOI：10.1042/bj1870281 · 被引用次数：13 · 研究领域：Silk-based biomaterials and applications、Skin and Cellular Biology Research、Enzyme Production and Characterization

Mouse epidermal keratin proteins were purified and labelled with 125I by chemical techniques. A radioimmunoassay method was established with a rabbit antibody elicited against the mouse keratin. This assay method was utilized to quantify keratin proteins of mouse and human epidermal extracts, both from intact tissues and cells in culture. As little as 30 ng of mouse keratin (0.5 pmol) was quantifiable.