Structure, tissue-specific expression, and transforming activity of the mouse met protooncogene.

作者：Anand S. Iyer, Thomas E. Kmiecik, M Park, Ira Owen Daar, Donald G. Blair, KJ Dunn, Pramod Sutrave, James N. Ihle, Myriam Bodescot, G Vande Woude · 发表于：PubMed · 年份：1990 · 被引用次数：118 · 研究领域：Epigenetics and DNA Methylation、Genetics and Neurodevelopmental Disorders、Pancreatic function and diabetes

A 6.7-kilobase met complementary DNA (cDNA) was isolated from a pcD cDNA library prepared from C3H mouse fibroblast cell line polyadenylated RNA. Sequence analysis of 6.7-kilobase met cDNA insert revealed that it contained the entire open reading frame and shared an overall homology of 88.1% with the human met gene. Using the mouse met cDNA as probe, high levels of met expression were observed in the kidney, brain, lung, skin, and embryonic tissue as well as in several factor responsive mouse myeloid cell lines. Under SV40 promoter control, the mouse met protooncogene cDNA in the pCD vector was able to transform NIH 3T3 cells. These transformed cells possess multiple copies of mouse met cDNA and exhibit properties of malignant cells, including growth in soft agar and induction of tumors in nude mice. Tumor explant cell lines analyzed by Western blot also reveal the presence of high levels of Mr 170,000 and 140,000 met protein product(s).