Epithelial cell-derived micro RNA-146a generates interleukin-10-producing monocytes to inhibit nasal allergy

作者：Xi Luo, Miaomiao Han, Liu Jianqi, Yu Wang, Xiang‐Qian Luo, Jing Zheng, Shuai Wang, Zhigang Liu, Dabo Liu, Ping–Chang Yang, Huabin Li · 发表于：Scientific Reports · 年份：2015 · DOI：10.1038/srep15937 · 被引用次数：37 · 研究领域：IL-33, ST2, and ILC Pathways、Extracellular vesicles in disease、Allergic Rhinitis and Sensitization

The aberrant immunity plays an important role in the pathogenesis of allergic diseases. Micro RNAs (miR) are involved in regulating the immunity in the body. This study aims to test a hypothesis that miR-146a induces the expression of interleukin (IL)-10 in monocytes (Mos). In this study, the levels of miR-146a were determined by real time RT-PCR. The IL-10(+) Mos were evaluated by flow cytometry. The miR-146a-laden exosomes were generated with RPMI2650 cells (an airway epithelial cell line). An allergic rhinitis mouse model was developed. The results showed that nasal epithelial cells expressed miR-146a, which was markedly lower in the nasal epithelial cells of patients with nasal allergy than that in healthy controls. Exposure to T helper (Th)2 cytokines suppressed the levels of miR-146a in the nasal epithelial cells. The nasal epithelial cell-derived miR-146a up regulated the expression of IL-10 in Mos. The inducible IL-10(+) Mos showed an immune suppressor effect on the activities of CD4(+) effector T cells and the Th2 polarization in a mouse model of allergic rhinitis. In summary, nasal epithelial cells express miR-146a, the latter is capable of inducing IL-10 expression in Mos, which suppress allergic reactions in the mouse nasal mucosa.