Extraction Procedures for Use Prior to HPLC Nucleotide Analysis Using Microparticle Chemically Bonded Packings
作者:S.-C. Chen, Pamela R. Brown, Douglas M. Rosie · 发表于:Journal of Chromatographic Science · 年份:1977 · DOI:10.1093/chromsci/15.6.218 · 被引用次数:124 · 研究领域:Analytical Chemistry and Chromatography、Microfluidic and Capillary Electrophoresis Applications、Advanced biosensing and bioanalysis techniques
In preparing nucleotide extracts for HPLC analysis using microparticle, chemically bonded packings, tri-octyl-amine-freon solutions can be used for the extraction of the acid from the solution if the concentration of the amine is carefully controlled.