Ebola RNA Persistence in Semen of Ebola Virus Disease Survivors — Final Report

作者：Gibrilla F. Deen, Nathalie Broutet, Wenbo Xu, Barbara Knust, Foday R. Sesay, Suzanna L.R. McDonald, Elizabeth Ervin, Jaclyn E. Marrinan, Philippe Gaillard, Ndema Habib, Hongtu Liu, William Liu, Anna E. Thorson, Francis Yamba, Thomas A. Massaquoi, Faustin James, Archchun Ariyarajah, Christine Ross, Kyle Bernstein, Antoine Coursier, John Klena, Marylin Carino, Alie H. Wurie, Yong Zhang, Marion S. Dumbuya, Neetu Abad, Baimba Idriss, Teodora Wi, Sarah D. Bennett, Tina Davies, Faiqa K. Ebrahim, Elissa Meites, Dhamari Naidoo, Samuel J. Smith, Patricia Ongpin, Tasneem Malik, Anshu Banerjee, Bobbie R. Erickson, Yongjian Liu, Yang Liu, Ke Xu, Aaron Brault, Kara N. Durski, Jörn Winter, Tara Sealy, Stuart T. Nichol, Margaret Lamunu, James Bangura, Sihem Landoulsi, Amara Jambai, Oliver Morgan, Guizhen Wu, Mifang Liang, Qiudong Su, Yu Lan, Yanzhe Hao, Pierre Formenty, Ute Ströher, Foday Sahr · 发表于：New England Journal of Medicine · 年份：2015 · DOI：10.1056/nejmoa1511410 · 被引用次数：379 · 研究领域：Viral Infections and Outbreaks Research、COVID-19 Impact on Reproduction、Disaster Response and Management

BACKGROUND: Ebola virus has been detected in the semen of men after their recovery from Ebola virus disease (EVD). We report the presence of Ebola virus RNA in semen in a cohort of survivors of EVD in Sierra Leone. METHODS: We enrolled a convenience sample of 220 adult male survivors of EVD in Sierra Leone, at various times after discharge from an Ebola treatment unit (ETU), in two phases (100 participants were in phase 1, and 120 in phase 2). Semen specimens obtained at baseline were tested by means of a quantitative reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP and VP40 (in phase 1) or NP and GP (in phase 2). This study did not evaluate directly the risk of sexual transmission of EVD. RESULTS: Of 210 participants who provided an initial semen specimen for analysis, 57 (27%) had positive results on quantitative RT-PCR. Ebola virus RNA was detected in the semen of all 7 men with a specimen obtained within 3 months after ETU discharge, in 26 of 42 (62%) with a specimen obtained at 4 to 6 months, in 15 of 60 (25%) with a specimen obtained at 7 to 9 months, in 4 of 26 (15%) with a specimen obtained at 10 to 12 months, in 4 of 38 (11%) with a specimen obtained at 13 to 15 months, in 1 of 25 (4%) with a specimen obtained at 16 to 18 months, and in no men with a specimen obtained at 19 months or later. Among the 46 participants with a positive result in phase 1, the median baseline cycle-threshold values (higher values ind...