Analysis of Differentiating Clones Derived from Marrow

作者：James E. Till, E. A. McCulloch, Robert A. Phillips, Louis Siminovitch · 发表于：Cold Spring Harbor Symposia on Quantitative Biology · 年份：1967 · DOI：10.1101/sqb.1967.032.01.058 · 被引用次数：21 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Glycosylation and Glycoproteins Research、Immunodeficiency and Autoimmune Disorders

Immunoglobulin-producing cells appear to be restricted in the number of specific antibodies they can produce (Attardi et al., 1959; Nossal and Lederberg, 1958). A central problem in cellular immunology is to determine the origin of this restriction in specificity. One approach is to con-sider the immune response as an example of cellular differentiation i which progenitor cells proliferate and differentiate, giving rise to function-ally mature, antibody-producing cells (see, e.g., reviews by Nossat, 1962; Talmage and Claman, 1964; Makinodan and Albright, 1967). From this point of view, one may ask whether the restriction in specificity is present in the progenitor or stem cells of the system, or whether it is imposed uring the process of cellular differentiation. If restriction of specificity exists in the stem cells, each clone derived from such a cell would also contain cells restricted in the number of specific antibodies they could produce. However, if specificity is acquired during the growth of such clones from previously uncommitted stem cells, then cells producing anti-bodies of different specificities might be contained within each clone. To examine these problems it is, therefore, necessary to obtain clones derived from stem cells of the immune system and to examine their cellular composition. In this paper we will describe a model system which we are using in an attempt to achieve this objective.