Gold Nanoparticle-Enabled Real-Time Ligation Chain Reaction for Ultrasensitive Detection of DNA

作者：Wei Shen, Huimin Deng, Zhiqiang Gao · 发表于：Journal of the American Chemical Society · 年份：2012 · DOI：10.1021/ja306265n · 被引用次数：144 · 研究领域：Advanced biosensing and bioanalysis techniques、Gold and Silver Nanoparticles Synthesis and Applications、Biosensors and Analytical Detection

A simple and ultrasensitive colorimetric DNA assay based on the detection of the product of a ligation chain reaction (LCR) and the use of gold nanoparticles (AuNPs) as signal generators has been developed. During LCR, the AuNPs were ligated together, resulting in a distinct color change in real time after a sufficient number of thermal cycles. The cumulative nature of the protocol produced a detection limit of 20 aM with a selectivity factor of 10(3).