Equivalence between Vaccinia Particles Counted by Electron Microscopy and Infectious Units of the Virus

作者：John R. Overman, Igor Tamm · 发表于：Experimental Biology and Medicine · 年份：1956 · DOI：10.3181/00379727-92-22621 · 被引用次数：26 · 研究领域：Poxvirus research and outbreaks、Bacteriophages and microbial interactions、Virus-based gene therapy research

1. Studies of the relation between the number of infectious or pock-forming units of vaccinia virus and the number of virus particles indicate a ratio of 1.5:1 under conditions described. 2. A modified centri-fugation cell is described with which virus suspensions can be concentrated and the particles then counted by electron microscopy. The virus particles are sedimented directly onto coated electron microscope grids which when removed from the centrifugation cell are ready for shadow-casting and examination. The precision of this method is comparable to that obtained by other technics.