Chronic effects of complete limbic lobe destruction in man

作者：Harvey M. Friedman, Norman Allen · 发表于：Neurology · 年份：1969 · DOI：10.1212/wnl.19.7.679 · 被引用次数：38 · 研究领域：Neurogenesis and neuroplasticity mechanisms

We directly isolated neural stem cells and lineage-restricted neuronal and glial progenitors from the embryonic rat telencephalon using a novel strategy of surface labeling and fluorescence-activated cell sorting. Neural stem cells, which did not express surface epitopes characteristic of differentiation or apoptosis, were sorted by negative selection. These cells predominantly expressed fibroblast growth factor receptor type 1 (FGFR-1), and a minority exhibited basic fibroblast growth factor (bFGF), whereas few expressed epidermal growth factor receptor (EGFR) or EGF. Clonal analyses revealed that these cells primarily self-renewed without differentiating in bFGF-containing medium, whereas few survived or expanded in EGF-containing medium. Culturing of neural stem cells in bFGF- and EGF-containing medium permitted both self-renewal and differentiation into neuronal, astroglial, and oligodendroglial phenotypes. In contrast, lineage-restricted progenitors were directly sorted by positive selection using a combination of surface epitopes identifying neuronal or glial phenotypes or both. These cells were also primarily FGFR-1+, with few EGFR+, and most expanded and progressed along their expected lineages in bFGF-containing medium but not in EGF-containing medium. Ca2+ imaging of self-renewing neural stem cells cultured in bFGF-containing medium revealed that bFGF, but not EGF, induced cytosolic Ca2+(Ca2+c) responses in these cells, whereas in bFGF- and EGF-containing medium, bo...