Enzyme-Free Amplified Detection of DNA by an Autonomous Ligation DNAzyme Machinery

作者：Fuan Wang, Johann Elbaz, Itamar Willner · 发表于：Journal of the American Chemical Society · 年份：2012 · DOI：10.1021/ja300616w · 被引用次数：116 · 研究领域：Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection、DNA and Nucleic Acid Chemistry

The Zn(2+)-dependent ligation DNAzyme is implemented as a biocatalyst for the amplified detection of a target DNA by the autonomous replication of a nucleic acid reporter unit that is generated by the catalyzed ligation process. The reporter units enhance the formation of active DNAzyme units, thus leading to the isothermal autocatalytic formation of the reporter elements. The system was further developed and applied for the amplified detection of Tay-Sachs genetic disorder mutant, with a detection limit of 1.0 × 10(-11) M. Besides providing a versatile paradigm for the amplified detection of DNA, the system reveals a new, enzyme-free, isothermal, autocatalytic mechanism that introduces means for effective programmed synthesis.