Further Characterization and Evidence for a Precursor in the Formation of Plasma Antinatriferic Factor

作者：Kenneth A. Gruber, Vardaman M. Buckalew · 发表于：Experimental Biology and Medicine · 年份：1978 · DOI：10.3181/00379727-159-40371 · 被引用次数：41 · 研究领域：Protein purification and stability、Monoclonal and Polyclonal Antibodies Research、Viral Infectious Diseases and Gene Expression in Insects

Antinatriferic factor was isolated from VE dog plasma on high pressure liquid chromatography. The use of an enzyme inhibitor while collecting plasma reduced the presence of this factor. A reverse-phase chromatography peptide map revealed 2 peptides whose presence was directly correlated with antinatriferic activity. The results suggest that antinatriferic factor is a small acidic peptide, formed from a precursor molecule. Reverse-phase chromatography may prove to be a chemical assay for antinatriferic factor.