Exploitation and Application of a Highly Sensitive Ru(II) Complex-Based Phosphorescent Chemodosimeter for Hg2+ in Aqueous Solutions and Living Cells

作者：Jiaxi Ru, Xiaoliang Tang, Zhenghua Ju, Guolin Zhang, Wei Dou, Xiangquan Mi, Chunming Wang, Weisheng Liu · 发表于：ACS Applied Materials & Interfaces · 年份：2015 · DOI：10.1021/am508484q · 被引用次数：36 · 研究领域：Molecular Sensors and Ion Detection、Analytical Chemistry and Sensors、Advanced biosensing and bioanalysis techniques

A novel Ru(II) complex-based phosphorescent probe Rubpy-1 was designed and synthesized conveniently by incorporating of chemodosimeter into the luminophor, which exhibits good water solubility, longer excitation wavelength, and rapid turn-on phosphorescent response only toward Hg(2+) in aqueous system under physiological pH. The spectral response mechanism and Hg(2+)-promoted structure change of the chemodosimeter were analyzed in detail by theoretical calculations and electrospray ionization mass spectrometry. When time-resolved photoluminescence techniques were used, the Rubpy-1 could eliminate effectively the signal interference from the short-lived background fluorescence in complicated media, accompanied by the significant improvement of the signal-to-noise ratio and the accuracy of the detection. Furthermore, Rubpy-1 showed low cytotoxicity and excellent membrane permeability toward living cells, which was successfully applied to monitor intracellular Hg(2+) effectively by confocal luminescence imaging.