Accumulation and Molecular Regulation of Anthocyanin in Purple Tumorous Stem Mustard (Brassica junceavar.tumidaTsen et Lee)

作者：Qiaoli Xie, Zongli Hu, Yanjie Zhang, Shibing Tian, Zhijin Wang, Zhiping Zhao, Yang Yang, Guoping Chen · 发表于：Journal of Agricultural and Food Chemistry · 年份：2014 · DOI：10.1021/jf501790a · 被引用次数：76 · 研究领域：Plant Gene Expression Analysis、Plant biochemistry and biosynthesis、Phytochemicals and Antioxidant Activities

Tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee) is an economically and nutritionally important dietary vegetable in Asian countries. Purple tumorous stem mustard contains inflated tumorous stem and abundant anthocyanin accumulation in leaves. Here, 20 anthocyanins were separated and identified from the purple tumorous stem mustard by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). In order to investigate the regulatory anthocyanin production in purple tumorous stem mustard, the expression of anthocyanin biosynthetic and regulatory genes in leaves from purple and green cultivars were examined. Regulatory gene BjTT8 and all biosynthetic genes were dramatically upregulated in the purple variety. Moreover, the transcript level of BjTT8 and all structural genes, except BjPAL, were all significantly higher in light-treated sprouts than in the dark. These results indicate that transcriptional activation of BjTT8 is associated with upregulation of most anthocyanin biosynthetic genes, to produce anthocyanins in purple tumorous stem mustard.