Proteomic analysis of heterosis in the leaves of sorghum–sudangrass hybrids

作者：Pingan Han, Xiaoping Lü, Fugui Mi, Jing Dong, Chunlei Xue, Jianke Li, Bin Han, Xiaoyu Zhang · 发表于：Acta Biochimica et Biophysica Sinica · 年份：2016 · DOI：10.1093/abbs/gmv126 · 被引用次数：15 · 研究领域：Genetic Mapping and Diversity in Plants and Animals、Bioenergy crop production and management、Ruminant Nutrition and Digestive Physiology

Sorghum-sudangrass hybrids are widely used for forage and silage in the animal husbandry industry due to their hardiness. The heterozygous first generation of sorghum-sudangrass hybrids displays performance superior to their homozygous, parental inbred lines. In order to study the molecular details underlying its heterosis, the leaves of sorghum-sudangrass hybrids and their parents were compared using mass spectrometry-based proteomics. Results showed that among the 996 proteins that were identified, 32 proteins showed 'additive accumulation expression patterns', indicating that the protein abundance in sorghum-sudangrass hybrids showed no significant difference from the average of their parents. Additionally, 74 proteins showed 'nonadditive accumulation expression patterns' (the proteins abundance in the hybrids showed significant difference from the average of their parents). Both additive and nonadditive proteins were mainly involved in photosynthesis and carbohydrate metabolism. More upregulated additive and nonadditive proteins were in the hybrids than in their parents, suggesting that additive and nonadditive proteins are essential to the vigor of sorghum-sudangrass hybrids. The nonadditive proteins were enriched in photosynthesis, carbohydrate metabolism, and protein oligomerization, but the additive proteins were not enriched in any pathway, which indicated that the nonadditive proteins could be greater contributors to heterosis than additive proteins. Furthermore, th...