Simultaneous determination of retinol, tocopherols, carotenes and lycopene in plasma by means of high-performance liquid chromatography on reversed phase.

作者：D Hess, Heidi Keller, B Oberlin, Riccardo Bonfanti, Willy Schüep · 发表于：PubMed · 年份：1991 · 被引用次数：283 · 研究领域：Antioxidant Activity and Oxidative Stress、Retinoids in leukemia and cellular processes

The determination of the vitamins A and E as well as of carotenes and lycopene is important for studies of cardiovascular diseases and cancer. A method for laboratory routine is reported to separate simultaneously retinol, tocopherols, alpha- and beta-carotene, lycopene and beta-cryptoxanthin in human plasma or serum by HPLC on reversed phase starting from one extract. Two detectors with programmable wavelength are used sequentially, a spectrophotometer for the detection of the carotenoids in the visible region and a fluorometer for the assay of retinol and the tocopherols.