Infectious clones of the crinivirus cucurbit chlorotic yellows virus are competent for plant systemic infection and vector transmission

作者：Yan Shi, Yajuan Shi, Qinsheng Gu, Fengming Yan, Xinyan Sun, Honglian Li, Linlin Chen, Bingjian Sun, Zhenyue Wang · 发表于：Journal of General Virology · 年份：2016 · DOI：10.1099/jgv.0.000453 · 被引用次数：18 · 研究领域：Plant Virus Research Studies、Insect-Plant Interactions and Control、Plant Parasitism and Resistance

Cucurbit chlorotic yellows virus (CCYV), a recently identified bipartite crinivirus, causes economic losses in cucurbit plants. CCYV is naturally transmitted only by whitefly Bemisia tabaci. Here we constructed full-length cDNA clones of CCYV (RNA1 and RNA2) fused to the T7 RNA polymerase promoter and the cauliflower mosaic virus 35S promoter. CCYV replicated and accumulated efficiently in Cucumis sativus protoplasts transfected with in vitro transcripts. Without RNA2, RNA1 replicated efficiently in C. sativus protoplasts. Agroinoculation with the infectious cDNA clones of CCYV resulted in systemic infection in the host plants of C. sativus and Nicotiana benthamiana. Virus derived from the infectious clones could be transmitted between cucumber plants by vector whiteflies. This system will greatly enhance the reverse genetic studies of CCYV gene functions.