An Interface for a Fragment Assembly Kernel

作者：Susan Mau Larson, Mudita Jain, Eric L. Anson, Eugene Wimberly Myers · 年份：1996 · 被引用次数：8 · 研究领域：Genomics and Phylogenetic Studies、Scientific Computing and Data Management、RNA and protein synthesis mechanisms

This document describes the C programming language interface to our Fragment Assembly Kernel. Inputs to the Fragment Assembly Kernel are (1) DNA fragment sequences from potentially inaccurate sequencing experiments, and (2) optional constraints on fragment assembly such as known fragment overlaps or relative fragment orientation. Fragment sequence version control is supported. The Fragment Assembly Kernel produces the most probable reconstructions of the original DNA sequence from the fragments, subject to any specified constraints. Each fragment assembly includes multiple sequence alignment and consensus sequences. Multiple sequence alignment editing capabilities are provided to allow manual correction of sequence errors.