Epithelial Cell Cultures From Normal and Cancerous Human Tissues2

作者：R. B. Owens, Helene S. Smith, Walter A. Nelson‐Rees, Emely Springer · 发表于：JNCI Journal of the National Cancer Institute · 年份：1976 · DOI：10.1093/jnci/56.4.843 · 被引用次数：181 · 研究领域：Cancer Cells and Metastasis、Cancer Research and Treatments

Thirty epithelial cell strains were isolated from human carcinomas and normal epithelial tissues by collagenase digestion and selective removal of fibroblasts with trypsin-Versene. Most strains were obtained from metastatic carcinomas or epithelia of the urinary and intestinal tracts. The success rate for growth of both neoplastic and normal tissues (excluding skin) was 38%. Six of these strains showed gross morphologic and chromosome changes typical of malignant cells. Nine resembled normal epithelium. The other 15 exhibited some degree of morphologic change from normal.