Effects of vagus nerve stimulation via cholinergic anti-inflammatory pathway activation on myocardial ischemia/reperfusion injury in canine.

作者：Rong Zhang, Najina Wugeti, Juan Sun, Yan Huang, Yujun Guo, Ling Zhang, Mei Ma, Xingui Guo, Changan Jiao, Wenli Xu, Tianqi Li, Haili Liu, Yi‐Tong Ma · 发表于：PubMed · 年份：2014 · 被引用次数：27 · 研究领域：Vagus Nerve Stimulation Research、Neuroinflammation and Neurodegeneration Mechanisms、Acupuncture Treatment Research Studies

BACKGROUND: Acute myocardial infarction (AMI) was a type of disease with high mortality rate and high disability rate. And about 50% of the final area of myocardial infarction after AMI was led by ischemia/reperfusion (I/R) injury. The I/R injury was a kind of systemic inflammatory response, in which the main performance laid in the release of the large quantity of inflammatory cytokines. The basic experiments, clinical studies and the large scaled epidemiology investigations found that the low functions of vagus nerves had close relevance with the occurrence, development and prognosis of the cardiovascular diseases. This study investigate the effects of cholinergic anti-inflammatory pathway with with vagus never stimulation I/R injury in canine. METHODS: 18 adult mongrel dogs were randomly divided into 3 groups (n = 6): sham operation group (sham Group), ischemia/reperfusion group (I/R group), right vagus nerve stimulation and ischemia/reperfusion group (STM group). The hemodynamic indexes were measured after reperfusion 120 min. Through internal jugular venous blood, serum acetylcholine (Ach), tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) concentrations were detected by ELISA. Alpha 7 subunit Ach acetylcholine receptor (α7nAchR) expression level was detected with immunohistochemical method. HE staining was used to observe the degree of neutrophil infiltration. RESULTS: After ischemia/reperfusion 120 min, compared with sham group, TNF-α and IL-6 were significa...