Effects of Autologous Bone Marrow Mesenchymal Stem Cell Transplantation on Angiogenesis in Rabbit Ischemic Limbs A high-frequency Ultrasound Evaluation

作者：Dong-Xiao Zhu, Zongning Miao, Jidong Zhao, Han-Guang Qian, Xiaoming Liu · 年份：2009 · 研究领域：Angiogenesis and VEGF in Cancer、Peripheral Artery Disease Management、Mesenchymal stem cell research

BACKGROUND: Several studies have demonstrated that stem cells can differentiate into vascular endothelial cells, and then further differentiate and form blood capillary. Based on this principle, autologous bone marrow mesenchymal stem cell (BMSC) transplantation promotes angiogenesis to treat ischemia in lower limb. OBJECTIVE: To evaluate the angiogenesis in rabbit ischemic limbs following autologous BMSC transplantation using high-frequency two-dimensional ultrasound detection in conjunction with Doppler color-flow imaging examination. DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed in the Third People's Hospital of Wuxi between March 2007 and April 2008. MATERIALS: Twenty-four New Zealand rabbits were randomized to a control group and a cell transplantation group, with 12 rabbits in each. METHODS: Ischemia in lower limbs was induced in all rabbits. One week following ischemia induction, the cell transplantation group was injected with 0.5 mL cell suspension, comprising 2×10^6 BrdU-labeled autologous BMSCs cultured in vitro, through multiple sites in the region of gastrocnemius muscle. Simultaneously, the control group received the same amount of physical saline in the same region. MAIN OUTCOME MEASURES: The initial segment of rabbit femoral artery and superficial femoral artery was subjected to high-frequency two-dimensional ultrasound and Doppler color-flow imaging examinations to measure femoral artery vascular internal diameter, b...