Nonenzymatic separation of myocardial cell nuclei from whole heart tissue

作者：C.C. Liew, George Jackowski, T. Ma, Michael J. Sole · 发表于：American Journal of Physiology-Cell Physiology · 年份：1983 · DOI：10.1152/ajpcell.1983.244.1.c3 · 被引用次数：147 · 研究领域：RNA and protein synthesis mechanisms、Protein Structure and Dynamics、RNA Research and Splicing

A simple and rapid nonenzymatic method has been developed to isolate myocardial cell nuclei from whole heart tissue. This method consists of a controlled disruption of cells followed by isopycnic gradient centrifugation. We have reviewed and compared our method to others more lengthy and laborious. By using a number of criteria, such as morphometric measurements, chemical composition, functional studies, specific nuclear protein markers, and mathematical analysis, we show that the nonenzymatic digestion method provides a most useful technique for the study of the biochemistry of the myocardial cell nucleus.