Analysis of Glutamic Acid in Cerebrospinal Fluid by Capillary Electrophoresis with High Frequency Conductivity Detection

作者：Hai Yun Zhai, Junmei Wang, Xiao Li Yao, Xue Cai Tan, Pei Xiang Cai, Zuan Guang Chen · 发表于：Chinese Chemical Letters · 年份：2005 · 被引用次数：5 · 研究领域：Metabolism and Genetic Disorders、Amino Acid Enzymes and Metabolism、Microfluidic and Capillary Electrophoresis Applications

A rapid method to determine glutamic acid (Glu) in cerebrospinal fluid (CSF) by capillary electrophoresis with high frequency conductivity detection (contactless conductivity detection) was described. The CSF sample was pretreated with silver cation resin to remove high concentration of Cl(superscript -) ions in CSF. The separation was achieved in the buffer solution of 10mmol/L Tris and 8mmol/L boric acid at the separation voltage of 20.0kV. Glu showed linear response in the range of 5.0×10^(-6) to 6.0×10^(-3)mol/L, the limit of detection was 10×10^(-6)mol/L. The method was used for analysis Glu in CSF satisfactorily with a recovery of 97.8-98.8%.