Accurate in vitro transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters.

作者：Daniel F. Bogenhagen, Barbara K. Yoza · 发表于：Molecular and Cellular Biology · 年份：1986 · DOI：10.1128/mcb.6.7.2543 · 被引用次数：44 · 研究领域：Mitochondrial Function and Pathology、RNA and protein synthesis mechanisms、RNA modifications and cancer

The mitochondrial RNA polymerase from Xenopus laevis oocytes was partially purified by heparin-Sepharose chromatography and phosphocellulose chromatography. This RNA polymerase preparation specifically initiated the transcription of X. laevis mitochondrial DNA (mtDNA) from two bidirectional promoters contained within a 123-base-pair segment of the mtDNA between the heavy-strand replication origin and the rRNA cistrons. Transcription in vitro initiated from precisely the same start sites previously mapped as initiation sites for transcription in vivo. At each of the four sites, initiation occurred within a conserved nucleotide sequence, ACPuTTATA. This consensus sequence is not related to promoters for transcription of human mtDNA.