Induced thiacloprid insensitivity in honeybees ( A pis mellifera L.) is associated with up‐regulation of detoxification genes

作者：Selcan Alptekin, Chris Bass, Colin Nicholls, Mark J. I. Paine, Suzanne J. Clark, L. M. Field, G. D. Moores · 发表于：Insect Molecular Biology · 年份：2016 · DOI：10.1111/imb.12211 · 被引用次数：57 · 研究领域：Insect and Pesticide Research、Plant and animal studies、Insect and Arachnid Ecology and Behavior

Honey bees, Apis mellifera, are markedly less sensitive to neonicotinoid insecticides containing a cyanoimino pharmacophore than to those with a nitroimino group. Although previous work has suggested that this results from enhanced metabolism of the former by detoxification enzymes, the specific enzyme(s) involved remain to be characterized. In this work, a pretreatment of honey bees with a sublethal dose of thiacloprid resulted in induced insensitivity to the same compound immediately following thiacloprid feeding. A longer pretreatment time resulted in no, or increased, sensitivity. Transcriptome profiling, using microarrays, identified a number of genes encoding detoxification enzymes that were over-expressed significantly in insecticide-treated bees compared with untreated controls. These included five P450s, CYP6BE1, CYP305D1, CYP6AS5, CYP315A1, CYP301A1, and a carboxyl/cholinesterase (CCE) CCE8. Four of these P450s were functionally expressed in Escherichia coli and their ability to metabolize thiacloprid examined by liquid chromatography-mass spectrometry (LC-MS) analysis.