E2F1 promotes tumor cell invasion and migration through regulating CD147 in prostate cancer

作者：Yuxiang Liang, Jianming Lü, Rujun Mo, Huichan He, Jian Xie, Funeng Jiang, Zhuoyuan Lin, Yanru Chen, Yongding Wu, Hongwei Luo, Zheng Luo, Weide Zhong · 发表于：International Journal of Oncology · 年份：2016 · DOI：10.3892/ijo.2016.3364 · 被引用次数：59 · 研究领域：Prostate Cancer Treatment and Research、RNA modifications and cancer、Molecular Biology Techniques and Applications

Increased expression of E2F1 has been reported to be associated with tumor growth and cell survival of prostate cancer (PCa). However, its roles and mechanisms on PCa have not been fully elucidated. The present study found that E2F1 overexpression in PCa tissues was significantly associated with high Gleason score (P=0.01) and advanced pathological stage (P=0.02). In addition, PCa patients with high E2F1 expression more frequently had shorter biochemical recurrence-free survival (P=0.047) than those with low E2F1 expression. Then, we confirmed that the knock-down of E2F1 expression was able to inhibit cell cycle progression, invasion and migration of PCa cell lines in vitro, along with tumor xenograft growth and epithelial-to-mesenchymal transition (EMT) in vivo. Moreover, we identified CD147 as a novel interaction partner for E2F1 through bio-informatic binding site prediction, combined with chromatin immunoprecipitation-PCR (ChIP-PCR) and western blot analysis. Taken together, our data delineate an as yet unrecognized function of E2F1 as enhancer of tumor invasion and migration of PCa via regulating the expression of CD147 in PCa. Importantly, E2F1 may function as a biomarker that can differentiate patients with biochemical recurrent and non-biochemical recurrent disease following radical prostatectomy, highlighting its potential as a therapeutic target.