Sca‐1 + mesenchymal stromal cells inhibit splenic marginal zone B lymphocytes commitment through Caspase‐3

作者：Yaozhen Chen, Jialei Yang, Huijie Zhang, Hong Fan, Ning An, Jiajia Xin, Na Li, Jinmei Xu, Wen Yin, Zhongliang Wu, Xingbin Hu · 发表于：Cell Biology International · 年份：2016 · DOI：10.1002/cbin.10591 · 被引用次数：7 · 研究领域：Mesenchymal stem cell research、Hematopoietic Stem Cell Transplantation、Immunotherapy and Immune Responses

Mesenchymal stromal cells (MSCs) have been characterized as an important component of hematopoietic niche, which are capable of modulating the immune system through interaction with a wide range of immune cells. Marginal zone B cells, one main type of mature B lymphocytes, play a central role in eliciting antibody response against pathogens. However, how MSCs and its subpopulations regulate marginal zone B cells commitment is unknown yet. In this study, we assessed the contribution of Sca-1(+) MSCs on marginal zone B cells commitment. Our results showed that Sca-1(+) MSCs inhibit the commitment of marginal zone B lymphocytes. The inhibition was exerted through lowered Caspase-3 expression. Furthermore, we found marginal zone B lymphocytes in spleen of Caspase-3 knockout mice decreased and Caspase-3 knockout Sca-1(+) MSCs accounted for the MZB lymphocytes decrease. In conclusion, our investigation provided clues about Sca-1(+) MSCs regulation on the commitment of marginal zone B cells through Caspase-3 gene.