Iron-binding fragments from the N-terminal and C-terminal regions of human lactoferrin

作者：Jeanne-Marie Bluard-Deconinck, Jonathan P. Williams, Robert W. Evans, Jacques Van Snick, Pierre A. Osinski, Pierre Masson · 发表于：Biochemical Journal · 年份：1978 · DOI：10.1042/bj1710321 · 被引用次数：45 · 研究领域：Infant Nutrition and Health、Pediatric Hepatobiliary Diseases and Treatments、Clinical Nutrition and Gastroenterology

Digestion of lactoferrin with pepsin at pH3.0 gave an iron-binding half-molecule that represents the C-terminal part of the native protein. Tryptic or chymotryptic digestion of 30%-iron-saturated lactoferrin yielded the N- and C-terminal half molecules, which could be separated by DEAE-Sephadex chromatography. The N- and C-terminal fragments did not show any immunological cross-reaction. The carbohydrate of lactoferrin was distributed equally between the two fragments.