Microsatellite Markers in White-Tailed Deer

作者：J. Andrew DeWoody, Rodney L. Honeycutt, Loren C. Skow · 发表于：Journal of Heredity · 年份：1995 · DOI：10.1093/oxfordjournals.jhered.a111593 · 被引用次数：161 · 研究领域：Genetic and phenotypic traits in livestock、Genetic diversity and population structure、Molecular Biology Techniques and Applications

A genomic library of DNA from white-tailed deer was constructed for the isolation of d(AC)n microsatellite repeats. PCR primers were designed from regions flanking each repeat and used to amplify DNA samples from a pedigreed herd of white-tailed deer and other artiodactyls. Allelic frequencies, PIC values, and heterozygosity values are reported for five polymorphic markers scored in 41 animals. Homologs of two of the five markers were assigned to bovine chromosomes 4 and 23, respectively, using a panel of bovine+hamster hybrid somatic cell lines.