The analysis of human serum lipoprotein distributions.

作者：A. M. Ewing, NORMAN K. FREEMAN, Frank T. Lindgren · 发表于：PubMed · 年份：1965 · 被引用次数：132 · 研究领域：Metabolomics and Mass Spectrometry Studies、Fatty Acid Research and Health、Adipose Tissue and Metabolism

Publisher Summary This chapter reviews distributions of human serum lipoproteins. Macromolecular distributions such as the human lipoprotein system have for many years been analyzed by utilizing the analytical ultracentrifuge. A biochemically important macromolecular system, exhibiting these special physical characteristics, is the low- and high-density lipoprotein spectrum of human sera. Although the analytical difficulties are considerable, ultracentrifugal analysis provides one of the few available approaches for an extensive quantitative analysis of such macromolecular distributions. Largely because of technical limitations, previous ultracentrifugal methodology for lipoprotein analysis has considered only broad subtractions of the low-density spectra. In the case of the high-density lipoprotein spectrum, this lipoprotein distribution has been divided essentially into two main classes on the basis of hydrated density below and above approximately 1.125 gm/ml, corresponding to high-density lipoprotein HDL 2 and HDL 3 , respectively. Because of the elaborate calculations and data processing required for this kind of mathematical analysis, it has not been feasible to consider potentially useful techniques to accomplish this task manually.