l -Cysteine improves antioxidant enzyme activity, post-thaw quality and fertility of Nili-Ravi buffalo ( Bubalus bubalis ) bull spermatozoa

作者：Sajid Iqbal, Amjad Riaz, S. M. H. Andrabi, Qaisar Shahzad, Aneela Zameer Durrani, Nasim Ahmad · 发表于：Andrologia · 年份：2016 · DOI：10.1111/and.12520 · 被引用次数：32 · 研究领域：Sperm and Testicular Function、Reproductive Biology and Fertility、Reproductive Physiology in Livestock

The effects of l-cysteine in extender on antioxidant enzymes profile during cryopreservation, post-thaw quality parameters and in vivo fertility of Nili-Ravi buffalo bull spermatozoa were studied. Semen samples from 4 buffalo bulls were diluted in Tris–citric acid-based extender having different concentrations of l-cysteine (0.0, 0.5, 1.0, 2.0 and 3.0 mm) and frozen in 0.5-ml French straws. The antioxidative enzymes [catalase, super oxide dismutase and total glutathione (peroxidase and reductase)] were significantly higher (P < 0.05) at pre-freezing and post-thawing in extender containing 2.0 mm l-cysteine as compared to other groups. Post-thaw total motility (%), progressive motility (%), rapid velocity (%), average path velocity (μm s−1), straight line velocity (μm s−1), curvilinear velocity (μm s−1), beat cross frequency (Hz), viable spermatozoa with intact plasmalemma (%), acrosome and DNA integrity (%) were higher with the addition of 2.0 mm l-cysteine as compared to other groups (P < 0.05). The fertility rates (59 versus 43%) were higher (P < 0.05) in buffaloes inseminated with doses containing 2.0 mm of l-cysteine than in the control. In conclusion, the addition of 2.0 mm l-cysteine in extender improved the antioxidant enzymes profile, post-thaw quality and in vivo fertility of Nili-Ravi buffalo bull spermatozoa.