Small-Molecule-Triggered and Light-Controlled Reversible Regulation of Enzymatic Activity

作者：Tian Tian, Yanyan Song, Jiaqi Wang, Boshi Fu, Zhiyong He, Xianqun Xu, Anling Li, Xin Zhou, Xin Zhou, Shaoru Wang, Xiang Zhou, Xiang Zhou · 发表于：Journal of the American Chemical Society · 年份：2016 · DOI：10.1021/jacs.5b11532 · 被引用次数：68 · 研究领域：Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry、Photochromic and Fluorescence Chemistry

The fine control of enzyme activity is essential for the regulation of many important cellular and organismal functions. The light-regulation of proteins serves as an important method for the spatiotemporal control over the production and degradation of an enzyme product. This area is of intense interest for researchers. To the best of our knowledge, the use of small molecules as light-triggered molecular switches to reversibly control enzyme activity at the protein level has not yet been studied. In the present study, we demonstrate the light-controlled reversible regulation of the enzyme using a small-molecule-triggered switch, which is based on molecular recognition between an azobenzene derivative and telomere DNA. This molecule interconverts between the trans and cis states under alternate 365 nm UV and visible light irradiation, which consequently triggers the compaction and extension of telomere DNA. We further provide direct evidence for this structural switch using a circular dichroism study. Furthermore, our strategy has been successfully used to effectively control blood clotting in human plasma.