The N-acetylation and estimation of hexosamines

作者：G. A. Levvy, Alexander B. McAllan · 发表于：Biochemical Journal · 年份：1959 · DOI：10.1042/bj0730127 · 被引用次数：351 · 研究领域：Glycosylation and Glycoproteins Research、Polyamine Metabolism and Applications、Cellular transport and secretion

Table 1.Moleculr weigl&8 of digestion products from rabbit y-globuline Rabbit y-globulin was prepared by sodium sulphate fractionation of serum (Kekwick, 1940).Refractive increment of protein Source Sample Buffer solution SS., , Molecular wt.± S.D. Rabbit y-globulin (batch 1) Whole globulin Phosphate (pH 6-8; I 0.2) 0-00149 6-50s 187 600±1400 Fraction I Phosphate (pH 6*8; 1 0.2) 0-00147 3-59s 49 600±1800 Rabbit y-globulin (batch 2) Fraction II Phosphate (pH 658; 1 0.2) 0-00138 3-55s 52 600±2600 Fraction III Acetate (pH 4*0; I 0 2) 0-00149 3 40s 80 200±9100 Fraction I Phosphate (pH 6-8; I 0.2) 0-00132 -50400±2000 Rabbit y-globulin (batch 3) Fraction II Phosphate (pH 658; I 0 2) 0-00118 65700±2000 Fraction m Aoetate (pH 4*5; I 0 2) 0-00133 -80 200±1600