Isolation, identification and characteristics of a difenoconazole-degrading bacterial strain B2

作者：Jinwei Zheng, Jian He, Yongsheng Wang, Ni Yingying, Shunpeng Li · 发表于：China Environmental Science · 年份：2009 · 被引用次数：6 · 研究领域：Mycotoxins in Agriculture and Food、Pesticide and Herbicide Environmental Studies、Viral Infectious Diseases and Gene Expression in Insects

A bacteria strain B2 capable of utilizing difenoconazole as sole carbon source was isolated from sludge collected from a pesticide manufacturer which has been producing difenoconazole for many years. It was identified as Ensifer sp. according to its physiological-biochemical properties and the analysis of its 16S rRNA gene sequence (GenBank Accession No. EU303300). Above 85% of 100mg/L difenoconazole was degraded by strain B2 within 24h. The optimal pH and temperature for degradation were 7.0 and 30~35℃ respectively. The degrading rate showed a positive correlation with the amount of initial inoculum size while a negative correlation with the initial concentration of difenoconazole. No degradation occurred if the initial concentration of difenoconazole excessed 400mg/L in mineral salt medium.