Identification of a Calmodulin-Dependent Protein Kinase in the Cardiac Cytosol, which Phosphorylates Phospholamban in the Sarcoplasmic Reticulum1

作者：Takafumi Iwasa, Nobuhiro Inoue, Eishichi Miyamoto · 发表于：The Journal of Biochemistry · 年份：1985 · DOI：10.1093/oxfordjournals.jbchem.a135313 · 被引用次数：18 · 研究领域：Cardiac electrophysiology and arrhythmias、Ion channel regulation and function、Cardiomyopathy and Myosin Studies

A multifunctional calmodulin-dependent protein kinase in the canine cardiac cytosol was purified to near homogeneity. The purified enzyme inactivated glycogen synthase by means of phosphorylation. The enzyme also phosphorylated phospholamban and several other proteins. In view of its physicochemical properties and substrate specificity, the enzyme differed from myosin light chain kinase and phosphorylase kinase, and was considered to belong to a class of similar calmodulin-dependent protein kinases from brain, liver, and skeletal muscle. The results suggest that the enzyme mediates multiple Ca2+-dependent functions in the heart.